Deanna’s Wolbachia Results

September 7, 2023

Sample information
Picture
Photos by: Deanna D.
Location
Collection date 09/14/2021
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Small, green, winged insect
Putative identification Arthropoda Hexapoda Insecta Hemiptera Cicadellidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The actual results from this experiment cannot be determined due to no bands visible in the control wells; wells three and five. If the controls had worked, the conclusion would be that the arthropod tested did not contain Wolbachia, due to only one 708bp band appearing in the sample lane.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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