Palm Beach Gardens—The Basketball Beetle

October 15, 2022

Sample information
Picture
Photos by: HunterW
Location
Collection date 09/18/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

The bug seemed to die from natural causes. When the bug was found, it was barely moving. I stored the bug in ethanol for two days prior to experiment.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Geotrupidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Protocol from DNA Extraction Qiagen Dneasy was followed. The only changes to procedure was that during the washing of DNA, AW1 was not used. AW2 was used twice to wash the DNA.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Confidence level is high due to the protocol that was followed step by step. There were very few mistakes made within the entire experimental procedure. The only change to the original procedure that instead of washing with AW1 and then AW2, AW2 was used for both washes.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Geotrupidae was found to be negative for Wolbachia.
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