|Captive / Cultivated?
|Palm Beach Atlantic University
|Arthropoda Hexapoda Insecta Coleoptera Geotrupidae
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
|Standard electrophoresis system
The protocol that was used is the DNA Extraction Qiagen Dneasy with a slight change when performing the washes, instead of using AW1, AW2 was used for both washes.
Lane 1: DNA Template
Lane 2: Insect- Hymenoptera
Lane 3: (+) control
Lane 4: (-) control
Lane 5: Water
|Explanation of confidence level
Confidence level is high because the protocol was followed step by step besides the wash part, which is not very likely to have an impact on the results obtained in the gel electrophoresis.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Geotrupidae was found to be negative for Wolbachia.