June Beetle

October 15, 2022

Sample information
Picture
Photos by: Danielle M., Carina G.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

Brown beetle with a hard shell. This sample has six legs. The sample also has a black line down its shell.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Only one sample arthropod was collected. We used 6X loading dye for the gel electrophoresis. The gel was run at 100V until the smallest visible fragment was about two thirds down the gel.

Lane 1 is the 100 bp DNA ladder, Lane 2 is arthropod sample 1, Lane 3 is positive arthropod control, Lane 4 is negative arthropod control, Lane 5 is positive DNA control, and Lane 6 is the water blank.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The positive arthropod control was amplified correctly and the 16s rRNA gene was visible on the gel and calculated to be 681 base pairs. The negative arthropod control CO1 gene was also visible on the gel at 395 base pairs. We also had a positive Wolbachia DNA control which was amplified properly and is 681 base pairs in length. The sample arthropod fragment matched the negative arthropod control fragment with a length of 681 base pairs.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post