Deepdigger Scarab Beetle

October 15, 2022

Sample information
Picture
Photos by: HunterW
Location
Collection date 09/18/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations
  1. The beetle was found in good anatomical conditions, seemed to have died die from natural causes, since when it was found it was barely moving. The bug was stored in ethanol for two days prior to experiment.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Geotrupidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

The protocol that was used is the DNA Extraction Qiagen Dneasy with a slight change when performing the washes, instead of using AW1, AW2 was used for both washes.

Lane 1: DNA Template

Lane 2: Insect- Hymenoptera

Lane 3: (+) control

Lane 4: (-) control

Lane 5: Water

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Confidence level is high because the protocol was followed step by step besides the wash part, which is not very likely to have an impact on the results obtained in the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Geotrupidae was found to be negative for Wolbachia.
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