|Captive / Cultivated?
|Palm Beach Atlantic University
Black beetle with striped or dotted pattern on exoskeleton. The superior portion of the insect has a midline and was collected and stored in ethanol one day prior to experimentation.
|Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Chrysomela Chrysomela vigintipunctata
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
|Standard electrophoresis system
Two wild-caught arthropods were collected. A 6X loading dye was used for the gel and run at 100V with the power supply. The order of samples from lanes 1-7 are as follows: 100bp ladder, Arthropod #1 (Willow Leaf Beetle), Arthropod #2 (White weevil), positive control, negative control, positive gDNA, and a water control.
|Explanation of confidence level
The protocol was followed and executed step by step with little to no error. For Lane 2, this simply gives us the insect DNA. The controls give a visual as to where the infected DNA would have ended if the insects did contain Wolbachia.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Chrysomela vigintipunctata was found to be negative for Wolbachia.