|Captive / Cultivated?||Wild-caught|
|Group||Palm Beach Atlantic University|
Black beetle with striped or dotted pattern on exoskeleton. The superior portion of the insect has a midline and was collected and stored in ethanol one day prior to experimentation.
|Putative identification||Hexapoda Insecta Coleoptera Chrysomelidae Chrysomela Chrysomela vigintipunctata|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
Two wild-caught arthropods were collected. A 6X loading dye was used for the gel and run at 100V with the power supply. The order of samples from lanes 1-7 are as follows: 100bp ladder, Arthropod #1 (Willow Leaf Beetle), Arthropod #2 (White weevil), positive control, negative control, positive gDNA, and a water control.
|Explanation of confidence level||
The protocol was followed and executed step by step with little to no error. For Lane 2, this simply gives us the insect DNA. The controls give a visual as to where the infected DNA would have ended if the insects did contain Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Chrysomela vigintipunctata was found to be negative for Wolbachia.|