Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/18/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | The bug seemed to die from natural causes. When the bug was found, it was barely moving. I stored the bug in ethanol for two days prior to experiment. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Geotrupidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Protocol from DNA Extraction Qiagen Dneasy was followed. The only changes to procedure was that during the washing of DNA, AW1 was not used. AW2 was used twice to wash the DNA. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Confidence level is high due to the protocol that was followed step by step. There were very few mistakes made within the entire experimental procedure. The only change to the original procedure that instead of washing with AW1 and then AW2, AW2 was used for both washes. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Geotrupidae was found to be negative for Wolbachia. |
Water Boatman