Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/18/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | I found the cockroach near my lights outside my house, it really disgusting but they like they keep themselves warm, so they find anything that has a warm surrounding. When I caught it, it was very easy but it felt disgusting. Rough from the bottom, feels spiky from the legs, Have an smooth texture from the top, looks disgusting. |
| Putative identification | |
Methods |
|
| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
DKH10
DKH 9
DKH8
DKH 7
DKH 6