Ant

April 2, 2023

Sample information
Picture
Photos by: Austin A. , Dawna W.
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Gel was moved a bit after having the DNA injected into the wells.
Used a 2% agarose gel instead of a 1%.
DNA ladder is in well 1, + DNA control is in well 2, water is in well 3, pillbug is in well 4, Wolbachia + is in well 5, Wolbachia – is in well 6, Ant is in well 7

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All positive and negative controls worked, and there is only one band which is closest to the Wolbachia – negative control band.
Wolbachia 16S sequence
5'CAT ACC TAT TCG AAG GGA TAG 3' Forward, 5' AGC TTC GAG TGA AAC CAA TTC 3' Reverse
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward, 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' Reverse
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be negative for Wolbachia.
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