DEMACASP23-8 Order: Hymenoptera

April 10, 2023

Sample information
Picture
Photos by: Samuel M
Location No map location data available.
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Taken from an ant hill beside the pond by the Georgia Southern University Armstrong campus.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The results showed correct information for the positive and negative controls. Therefore we can be confident that the negative Wolbachia results are valid.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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