NESACKSp23-18 Isoptera

April 13, 2023

Sample information
Picture
Photos by: Ishara D
Location
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found near a large point under a fallen, rotting tree branch that was slightly damp.
Putative identification Arthropoda Hexapoda Insecta Isoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I did need some help, but I was successfully able to extract DNA from my insect and proceed with the gel process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isoptera was found to be negative for Wolbachia.
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