KS MH – 1946

May 12, 2023

Sample information
Picture
Photos by: Kristin S., Melissa H., Michelle L., Shuai Z.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

The Cucline Mosquito had a slim body, long legs and shorter wings. The wings had a yellowish color and the body was a dark brown with black accents. The mosquito had a 2.2cm body. The arms and legs were hairy.
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

When examining our gel, there were barely any bands that appeared, but the DNA ladder appeared. We believe that our data is correct, though cannot be certain.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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