Brown Centipede

May 11, 2023

Sample information
Picture
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The assigned confidence level for the observation is medium due to the detection of bands, albeit of faint intensity. This determination is rooted in the understanding that band formations typically signify underlying patterns or structures within the observed data. However, the diminished prominence of these bands raises certain uncertainties, thereby warranting a moderate confidence level. In order to enhance the academic tone, it is important to emphasize the empirical evidence of the observed bands while acknowledging the limitations imposed by their faint appearance, ultimately leading to the medium confidence rating. DNA concentration being less then optimal may have also led to above results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chilopoda was found to be negative for Wolbachia.
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