Triangulate Cobweb Spider- Lauren (LP01)

June 7, 2023

Sample information
Picture
Photos by: Myself
Location
Collection date 05/30/2023
Captive / Cultivated? Wild-caught
Group Pingry School
Observations
  • Fast and jumpy
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
  • 100% same sequence to 16s sequence
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGA GCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTG GGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGT CGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGC TAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT TTCACTCGAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Unsuccessful PCR- see bottom half of gel
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Steatoda triangulosa was found to be postive for Wolbachia.
Report Inappropriate Post