Ant, Camponotus pennsylvanicus

November 26, 2023

Sample information
Picture
Location
Collection date
Captive / Cultivated?
Group Edmund Burke School
Observations

medium sized ant, was behind school running across pavement. Was about 80 degrees outside, sunny day. Last day of summer.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction, the lab went well with no struggles, protocol was followed, surfaces sterilized and pipette tips changed.

PCR: there were 2 issues with the PCR, first the proportions of compounds in the cocktail were off, but we remade the cocktail so this did not affect the results and the Wolbachia PCR was botched at first but we redid it and got conclusive results

Gel Electrophoresis, arthropod: got a clean bar for both Wolbachia and CO1 Arthropod gene. As for Wolbachia we at first had the issue of no bars, due to a PCR error, but we redid the PCR and Electrophoresis and got conclusive results

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The bar was nice and solid. The sequencing had a low e value as well which shows that the gene was most likely wolbachia
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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