Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/19/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Very small ant, was behind school running across pavement. Was about 80 degrees outside, sunny day. Was the last day of summer. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA Extraction, the lab went well with no struggles, protocol was followed, surfaces sterilized and pipette tips changed. PCR: there were 2 issues with the PCR, first the proportions of compounds in the cocktail were off, but we remade the cocktail so this did not affect the results and the Wolbachia PCR was botched at first but we redid it and got conclusive results Gel Electrophoresis, For the arthropod CO1 we got no bar, perhapse because the ant was too small and therefore the DNA sample was too small. As for Wolbachia we at first had the issue of no bars, due to a PCR error, but we redid the PCR and Electrophoresis and got conclusive results. This ant was positive for Wolbachia. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Because we could not extract enough DNA I have no idea whether there was Wolbachia or not |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
Water Boatman