Small ant E1

November 26, 2023

Sample information
Picture
Location
Collection date 09/19/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Very small ant, was behind school running across pavement. Was about 80 degrees outside, sunny day. Was the last day of summer.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction, the lab went well with no struggles, protocol was followed, surfaces sterilized and pipette tips changed.

PCR: there were 2 issues with the PCR, first the proportions of compounds in the cocktail were off, but we remade the cocktail so this did not affect the results and the Wolbachia PCR was botched at first but we redid it and got conclusive results

Gel Electrophoresis, For the arthropod CO1 we got no bar, perhapse because the ant was too small and therefore the DNA sample was too small. As for Wolbachia we at first had the issue of no bars, due to a PCR error, but we redid the PCR and Electrophoresis and got conclusive results. This ant was positive for Wolbachia.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Because we could not extract enough DNA I have no idea whether there was Wolbachia or not
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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