Daddy Long Legs

November 29, 2023

Sample information
Picture
Photos by: Jordyn Owens
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
Putative identification Arthropoda Chelicerata Arachnida Opiliones

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence level is in the middle because my line on the gel was faded a little.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Opiliones was found to be postive for Wolbachia.
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