Daddy Long Legs

Sample information

Photos by: Jordyn Owens
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Putative identification Arthropoda Chelicerata Arachnida Opiliones


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence level is in the middle because my line on the gel was faded a little.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Opiliones was found to be postive for Wolbachia.
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