Fire ant

Sample information

Picture
Photos by: Noah Carpenter
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
Putative identification Arthropoda Hexapoda Entognatha

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

The weather is 37 degrees.

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The true average number of fire ants pwr square meter in the entire forest falls withing the interval of 12 to 18 fire ants.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Entognatha was found to be postive for Wolbachia.
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