Fire ant

Sample information

Photos by: Noah Carpenter
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School

During my park exploration, I came across a log situated near a tree. I picked up the log with my bare hands and discovered a colony of fire ants actively moving in and out of an underground nest. The scene was a glimpse into the wild nature of these ants, showcasing their natural behaviors in their habitat. Motivated to capture one for closer observation, I retrieved a tube and carefully positioned it in the ground near the nest entrance. With precision, I observed as a fire ant walked into the tube. Seizing the moment, I closed the tube, successfully capturing the fire ant within.

Putative identification Arthropoda Hexapoda Entognatha


Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

The weather is 37 degrees.


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The true average number of fire ants per square meter in the entire forest falls withing the interval of 12 to 18 fire ants. Each of them was so wild and biology DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Entognatha was found to be postive for Wolbachia.
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