BroACHFall2023LTC-Odonata

November 8, 2023

Sample information
Picture
Photos by: Luci C.
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Method: sweep net

Location: mixed vegetation against wooden fence by police building on west side of campus

Time: 1:30-2pm

Environment: tall grass and weeds
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All controls worked/showed up efficiently. Detected CO1 gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be postive for Wolbachia.
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