Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Under trees, grass, & ground (near parking lot) • Canvas catching net method, forceps method, mat and beat stick method |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Insect sample is infected with Wolbachia. On the gel electrophoresis results, there were two bands for the positive control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be postive for Wolbachia. |
Water Boatman