IPACHF23

November 8, 2023

Sample information
Picture
Photos by: Ishika patel
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Under trees, grass, & ground (near parking lot)

• Canvas catching net method, forceps method, mat and beat stick method
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Insect sample is infected with Wolbachia. On the gel electrophoresis results, there were two bands for the positive control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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