Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | Basil plant housing hundreds of Glaber ants which seemed to be feeding on aphids living on basil. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Ochetellus Ochetellus glaber |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I used 4 different Ochetellus glaber samples in this reaction in the first four lanes. The upper portion of the gel screened for the PCR samples that used CO1 primers. The lower lanes used the samples that used Wolbachia primers during the PCR. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I forgot to use DNA positive control, however I did use a fruit fly that was infected with Wolbachia, and a fruit fly that was uninfected with Wolbachia. In the lower lanes you can see a positive result for my infected fruit fly control. So the controls did work, however the final DNA control was left out. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Ochetellus glaber was found to be negative for Wolbachia. |
Water Boatman