Ochetellus glaber (Glaber Ant)

November 21, 2023

Sample information
Picture
Photos by: Safir K.
Location
Collection date 09/07/2023
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Basil plant housing hundreds of Glaber ants which seemed to be feeding on aphids living on basil.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Ochetellus Ochetellus glaber

Methods
Extraction kit Edwards Buffer
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

I used 4 different Ochetellus glaber samples in this reaction in the first four lanes. The upper portion of the gel screened for the PCR samples that used CO1 primers. The lower lanes used the samples that used Wolbachia primers during the PCR.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I forgot to use DNA positive control, however I did use a fruit fly that was infected with Wolbachia, and a fruit fly that was uninfected with Wolbachia. In the lower lanes you can see a positive result for my infected fruit fly control. So the controls did work, however the final DNA control was left out.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ochetellus glaber was found to be negative for Wolbachia.
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