Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | My beetle was found under an old log. There were multiple beetles there but caught by different classmates. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I had to centrifuge my DNA solution 3 times in the PCR procedure due to the filter getting clogged and trying to get more liquid at the bottom of the tube. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | My confidence levels are in the medium area because once we were crushing the DNA and filtering it mine clogged the filter which most likely cause my results to be inconclusive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman