Collection of beetle for ISRII

November 29, 2023

Sample information
Picture
Photos by: Valentina C
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

My beetle was found under an old log. There were multiple beetles there but caught by different classmates.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

I had to centrifuge my DNA solution 3 times in the PCR procedure due to the filter getting clogged and trying to get more liquid at the bottom of the tube.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence levels are in the medium area because once we were crushing the DNA and filtering it mine clogged the filter which most likely cause my results to be inconclusive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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