Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | On top of a rock, the spider was frozen because of how cold it was outside. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Pholcidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low because with this lab, the steps were very important, and you had to follow them or you could mess up easily. So I don’t trust my results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman