Daddy Long Leg Spider

November 29, 2023

Sample information
Picture
Photos by: McKenzi S.
Location No map location data available.
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

On top of a rock, the spider was frozen because of how cold it was outside.
Putative identification Arthropoda Arachnida Araneae Pholcidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My confidence level is low because with this lab, the steps were very important, and you had to follow them or you could mess up easily. So I don’t trust my results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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