Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | Location: by a public restroom near a light |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Results are in the third column |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | We followed the protocol carefully |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be postive for Wolbachia. |
Water Boatman