March 1, 2024

Sample information
Picture
Photos by: Vincent S.
Location
Collection date 02/29/2024
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High
Observations

We observed the use of nanometers and the use of electrophoreses, we figured out how and what Wolbachia looks like in a mosquito.
Putative identification Arthropoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The line was there for most of the part but not a super definitive line.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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