Mosquito 1

March 1, 2024

Sample information
Picture
Photos by: Hannah Q.
Location
Collection date 08/01/2023
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High
Observations

Caught near a strand of mature red pine, white pine, oak, and cherry trees. The season was late summer. It was 70°.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was a band for the positive control and not one for my mosquito.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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