Melanoplus bruneri

April 28, 2024

Sample information
Picture
Photos by: Aaqib O
Location
Collection date 09/05/2023
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

“collected by Fall 2023 students”
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus Melanoplus bruneri

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence
Summary The Melanoplus bruneri was found to be postive for Wolbachia.
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