Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | River Valley Community College |
| Observations | Caught flying around in house. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Anopheles Anopheles punctipennis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | When prepping gel, we doubled the recipe from 0.2g of agarose & 20ml of buffer to 0.4g of agarose and 40ml of buffer. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Some controls worked but some did not. One of our positive controls did not work. Also, the mosquito did not test positive for arthropod DNA. Indicating that initial extraction did not go according to plan. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis