Tachypodoiulus niger LL2

Sample information

Picture
Photos by: L.L.
Location
Collection date 03/14/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Found in the forest, alone under a dead treetrunk.

Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae Tachypodoiulus Tachypodoiulus niger

Methods

Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident, because the cytochrome oxidase band (around 700) is seen, but no Wolbachia.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CTAACTGGAWGGTGCCGATGATGATAGGTGCGCCAGACATGGSGTTCCCTAGGCTGAACAACATGAGCTTTTGGTTGTTAATTCCTGCGGCTATCTTACTAGTGGTTTCTATTTTTGTACCAGGTGGGGCGATAGCRGGGGCTGGACAATGTATCCACCACTGTCTCTACAACAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tachypodoiulus niger was found to be negative for Wolbachia.
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