GRP 12 – Jumping Spider/Salticidae

May 23, 2024

Sample information
Picture
Photos by: Natalie A., Juan C., Analis G., Xenia M.
Location
Collection date 04/27/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

This jumping spider was caught as it crawled along a wall inside a household.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Ran gel for 60 minutes at 115 V and 60amps and visualized under UV light.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our confidence level that the jumping spider has Wolbachia because there is a matching band in lane 3(jumping spider) and lane 9(positive arthropod control) proving that there is a presence of Wolbachia in both.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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