GRP2 Coccinellidae #2

May 24, 2024

Sample information
Picture
Photos by: Kayla J.
Location
Collection date 05/13/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Caught in sparse, dry, and flat environments. The bug was crawling on a flower prior to capture. Temperature was roughly 64º-68ºF. The surrounding plants were dry bushes or short and stubby trees. Weeds also present.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit tweezers
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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