Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/17/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Collected Outside, 70ºF, wet ground, only one of its kind in the area. Terrestrial, urban habitiat. Yellow, spotted, small.
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| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Diabrotica Diabrotica undecimpunctata |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Crushed, extracted abdomen, no exoskeleton. PCR used to amplify the specific genes. Dyed the samples of DNA, mixed, and put into respective gel spots.
Arthropod CO1: No mistakes, controls worked to perfection. Gel electrophoresis: No mistakes, controls worked. 1-kb ladder 2- C-4 3- C-5 4- W+ 5- W- 6- H2O 7 – empty 8- empty 9 – rerun of C-3 PCR: No mistake |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Multiple tests showed no trace of Wolbachia gene, no errors made in setup or testing of this sample. Controls of W+, H2O, and W- all came out the way they were supposed to. No errors in the lead-up/process of this lab for this sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Diabrotica undecimpunctata
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Diabrotica undecimpunctata was found to be negative for Wolbachia. |
Water Boatman