Sample information

The Arthrpod was caught in a house’s basement. It is very large and features long, spindly legs. It looks kind of like a grasshopper. It was very large. It seemed to do very well with dark spaces in an urban house’s basement. It was fall, and the house was 75 degrees Fahrenheit.

Methods

DNA Extraction

1. The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.

PCR”:

1. There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.

Gel electrophoresis, Arthropod CO1

1. The ladder was marked on all Gels
2. Samples B-1 through B-6 were positive for arthropod DNA
3. The positive and negative controls were also marked positive and water was marked negative

Gel electrophoresis, Wolbachia

1. B-1 through B-5 were negative and B-6 was positive
2. The controls worked with both negative and water controls marked negative and the positive control marked positive
3. There were no notable mistakes or errors

Results

The gel came back very clear. There is no indication of Wolbachia. The positive control came back positive, while the negative control was negative. This indicates that everything went smoothly. There were some issues with contamination in B-2, B-3, B-4, B-5, and B-6, as the pipette tips were not properly changed. The gel ran flawlessly, so any contamination was minute.