|Captive / Cultivated?||Wild-caught|
|Group||St. John High School USD 350|
These fireflies were collected after dark in the grass.
|Putative identification||Hexapoda Insecta Coleoptera Lampyridae|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
The fireflies were stored in 90% ethanol for about six months prior to extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level||
All of the controls worked and the fireflies only had one band so I am confident they are negative for Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Lampyridae was found to be negative for Wolbachia.|