|Captive / Cultivated?
|St. John High School USD 350
These fireflies were collected after dark in the grass.
|Arthropoda Hexapoda Insecta Coleoptera Lampyridae
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
The fireflies were stored in 90% ethanol for about six months prior to extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level
All of the controls worked and the fireflies only had one band so I am confident they are negative for Wolbachia.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Lampyridae was found to be negative for Wolbachia.