Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found in forested, moist area under leaves and branches |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Wolbachia gel electrophoresis results were not clear, with bands being blurry and indistinct. The PCR Positive control was faint while the positive Drosophila control was nonexistent. Unclear results from the controls, likely due to poor DNA extraction, prevent accurate Wolbachia determination. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
(no title)
Arthropod Collection Lab
House Cricket
Week 2/18 lab – Arthropod Collection
Week 2/18 Bio Lab Arthropod Identification