MACALF24-SB Dermaptera

November 15, 2024

Sample information
Picture
Photos by: Stormee B.
Location
Collection date 08/29/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of day: 2:30pm – 3:10pm

Weather conditions: 99 degrees – High 90 degrees Low 76 degrees – 68% Humidity                 8 mph Wind – 20 Air Index

Used a tray and jar to collect the insect. Found in a semi-dry environment; in the woods near a pond. The earwig was collected from a fallen tree branch.
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that the results are correct due to the correctness of the positive, negative, and no template controls. I am also confident that there was no cross contamination between the two samples as they were both negative and that couldn’t be possible if one had  Wolbachia and there was contamination.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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