Armadillidiidae GRP12

May 27, 2025

Sample information
Picture
Photos by: Christian H.
Location
Collection date 05/13/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

caught deceased
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain Fast Blast
Gel images
Protocol notes

followed all of the lab instructions

lanes (from left to right):

  1. beetle
  2. Roly- Poly
  3. PCR Standard
  4. Arthropod Positive Control
  5. Arthropod Negative Control
  6. DNA Positive Control
  7. empty
  8. empty

 

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We are not fully confident in our results because there was some contamination found in our gel, and our PCR samples were not sent to the lab. We are at least a little confident that Wolbachia is found in this arthropod because the bands in both of our gels show similar bands (our controls successfully ran), and along with the research we did, we discovered that the pillbug is a common arthropod to carry Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be postive for Wolbachia.
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