Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/05/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | I found the boxelder bug on my brick wall house. I found it early in the morning like 6 or 7 . The bug was very tame went into the bag easily. |
| Putative identification | Arthropoda Insecta Hemiptera Rhopalidae |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | i am confident in these results because they were tested by vanderbilt university which is a very good school . |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Rhopalidae was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica