NV 41 Boxelder Bug

April 14, 2021

Sample information
Picture
Photos by: Rachel Lachenicht
Location
Collection date 10/05/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

I found the boxelder bug on my brick wall house. I found it early in the morning like 6 or 7 . The bug was very tame went into the bag easily.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Rhopalidae

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

i am confident in these results because they were tested by vanderbilt university which is a very good school .
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Rhopalidae was found to be negative for Wolbachia.
Report Inappropriate Post