Small Honey Ant

February 18, 2026

Sample information
Picture
Entry by: Sabrina D
Location
Collection date 10/03/2025
Captive / Cultivated? Wild-caught
Group Penn State University
Observations

This was the only ant that was on a rick and in some mulch, so I collected it. It was a clear day with little wind.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Prenolepis Prenolepis imparis

Methods
Extraction kit QIAGEN DNeasy kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system agarose gel electrophoresis
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

There was no problem with the protocol, so I am confident that my arthropod was not infected with Wolbachia.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There were no problems with the controls or the protocol, meaning that I am confident in my results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Prenolepis imparis was found to be negative for Wolbachia.
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