Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 12/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | The arthropod was found in a palm tree in California during December. The weather was around 60°F, which was surprising since grasshoppers are rarely seen during the winter. I found the grasshopper inside a crack in the middle of the tree trunk rather than in the leaves. The Arthropod was already dead, and I believe it likely died due to the cold weather.
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| Putative identification | Arthropoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level |
Our confidence level is moderate because the controls worked as expected and we followed the protocol closely. However, our results were ultimately inconclusive( unknown). This was likely due to an experimental error where the Arthropods hard shell wasn’t sufficiently crushed, preventing a clear reading. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
Blattodea LB2
tenebrio molitor_To1
JC2
YSD2