Grasshopper

March 25, 2026

Sample information
Picture
Entry by: Brianne S
Location
Collection date 11/10/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

My grasshopper was more of a red-ish tan color. After a couple of days, the red disappeared.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed MiniOne Protocol

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence level is medium because I believe we did not grind the DNA long enough for it to work. Our grasshopper and positive control band did not show up for the Wolbachia electrophoresis gel. So, we are unsure if our grasshopper has Wolbachia because the positive control didn’t work. Also, we added too much proteinase K to our positive and negative controls.

 

Wolbachia:                                      Arthropod:
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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