Sample information

Order: Isopod

Family: Armadillidiidae

Appearance: Big, Black, Oval-shaped exoskeleton body, 7 pairs of legs, 14 total

Behavior: Nocturnal, Thrive in dark, damp environments

Defense: Roll themselves into a ball as self-defense when threatened

Ecological Role: Assists in decomposition and returns nutrients for plants into the soil

Activity: Crawl around at night, in damp areas

Methods

DNA Extraction

We dissected the Rolly Polly by isolating its abdomen to crush it for our sample DNA extraction.

Gel Electrophoresis

The following lane order for our gel:

1. N/A
2. N/A
3. Ladder
4. Sample #1 – Moth
5. Sample #2 – Rolly Polly
6. Sample #3 – Fly
7. Sample #4 – Pincher
8. Sample #5 – Spider
9. Wolbachia Positive Control
10. Wolbachia Negative Control
11. Positive DNA
12. N/A
13. N/A
14. N/A
15. N/A

Results

Given that the first run of our gel electrophoresis resulted in invalid data, we had to rerun our samples in our to get valid results. Our confidence levels were low at first but once we ran out gel and got bands for all of our wells. We are more than confident that our results are reliable due to our extensive precautions that we took and the multiple trial and errors.