Sample information

Order: Lepidoptera

Family: Depends on species

Appearance:  fuzzy, large wings

Behavior: attracted to light, rest during the day

Defense: camouflage to hide from predators

Ecological Role: help pollinate plants and are food for other animals

Activity: active at night

Methods

DNA Extraction

We dissected the Moth by isolating its abdomen to crush it for our sample DNA extraction.

Gel Electrophoresis

The following lane order for our gel:

N/A
N/A
Ladder
Sample #1 – Moth
Sample #2 – Rolly Polly
Sample #3 – Fly
Sample #4 – Pincher
Sample #5 – Spider
Wolbachia Positive Control
Wolbachia Negative Control
Positive DNA
N/A
N/A
N/A
N/A

Results

Given that the first run of our gel electrophoresis resulted in invalid data, we had to rerun our samples in our to get valid results. Our confidence levels were low at first but once we ran out gel and got bands for all of our wells. We are more than confident that our results are reliable due to our extensive precautions that we took and the multiple trial and errors.