Sample information

I found this spider on the walls of my house, it had short fuzz on its body and was pretty small. It was the only spider I saw in my house and I haven’t seen another one like it before.

Methods

DNA Extraction: I cut off all the legs and used DNA from the abdomen, crushed it up really well.

The negative control in arthropod DNA showed up which means something went wrong during the protocol. Majority of our data did not result in visible bands which could also be due to a lack of Wolbachia or reagents that weren’t working properly.

Results

In our experiment, the negative control for the arthropod DNA unexpectedly showed a band, which invalidated the rest of our results. Additionally, none of our actual samples showed a clear band for arthropod DNA. Even though the Wolbachia positive and negative controls worked exactly as they were supposed to, the issues with the arthropod DNA make us uncertain about our overall findings.

For the fasta and ab1 upload, I’m not uploading anything because I didn’t get any viable DNA or Wolbachia levels from the gel electrophoresis.