Sample information

Photos by: Kavi A
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Putative identification Arthropoda Hexapoda


Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer PBS
DNA stain
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I am moderately confident because our positive DNA Extraction control never showed Wolbachia, yet all of our other controls were correct. Most class groups did not get any arthropod DNA at all. We believe this is because the insects wer enot crushed up enough pre-experiment.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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