Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/23/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Singapore American School |
| Observations | |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | PBS |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | I am moderately confident because our positive DNA Extraction control never showed Wolbachia, yet all of our other controls were correct. Most class groups did not get any arthropod DNA at all. We believe this is because the insects wer enot crushed up enough pre-experiment. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman