A.4.1

February 25, 2022

Sample information
Picture
Photos by: Kavi A
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations
Putative identification Arthropoda Hexapoda

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer PBS
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I am moderately confident because our positive DNA Extraction control never showed Wolbachia, yet all of our other controls were correct. Most class groups did not get any arthropod DNA at all. We believe this is because the insects wer enot crushed up enough pre-experiment.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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