Sample information |
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Picture |
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Location | |
Collection date | 09/13/2022 |
Captive / Cultivated? | Wild-caught |
Group | KantiWil |
Observations | Waldboden, auf steinigem Untergrund |
Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Myrmica Myrmica rubra |
Methods |
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Extraction kit | Instagene Matrix |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Auf dem Gel ist eine Bande von der Cytochromoxidase zu sehen. PCR hat Funktioniert |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download FASTA
Download AB1
TAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTT
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Myrmica rubra was found to be negative for Wolbachia. |