Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 09/17/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | Wasp was caught using plastic container, later frozen and immersed in alcohol in a test tube. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed LAB 2 and LAB 3 protocol provided by The Wolbachia Project |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | All our controls ran through the gel perfectly and provided great comparisons for samples. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Polistes fuscatus was found to be postive for Wolbachia. |
Water Boatman