Wolbachia Lab – Paper Wasp

October 17, 2022

Sample information
Picture
Photos by: Faith A.
Location No map location data available.
Collection date 09/17/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

Wasp was caught using plastic container, later frozen and immersed in alcohol in a test tube.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes fuscatus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Followed LAB 2 and LAB 3 protocol provided by The Wolbachia Project

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All our controls ran through the gel perfectly and provided great comparisons for samples.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polistes fuscatus was found to be postive for Wolbachia.
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