|Captive / Cultivated?||Captive / Cultivated|
|Group||South Plainfield High School|
Female larvae were captured by the Middlesex mosquito commission and then grown into adults before being donated to our class.
|Putative identification||Hexapoda Insecta Diptera Culicidae Culex|
|Extraction kit||qiagen blood and tissue|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
Modification P2 for ATL and N3 for AL
|Explanation of confidence level||
All procedure was followed correctly and through the gels, A strong band was shown at the 500 mark.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Culex was found to be postive for Wolbachia.|