Wolbachia #2: Ant

March 31, 2023

Sample information
Picture
Photos by: Julia B.
Location
Collection date 03/25/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Used a 2% gel.

Well 1: Ladder (100bp)

Well 2:  (+) DNA Control

Well 3: (-) Wolbachia

Well 4: (+) Wolbachia

Well 5:  arthropod #1 (ant)

Well 6: arthropod #2 (ant)

Well 7: (-) DNA Control/Water

Well 8: Nothing

Well 9:Nothing
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

There may have been a pipetting error, PCR error, or the sample may have not been entered into the gel electrophoresis well. There is no way to tell if the arthropod had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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