NESACBSp23-3-JB Coleoptera

April 10, 2023

Sample information
Picture
Photos by: JessicaB
Location
Collection date 01/29/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect Order: Coleoptera

Collection Date/time: January 29 at 10:30
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I did not have a band due to possible contamination or no DNA was extracted.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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